3 articles published in JoVE
Polysome Profiling in Leishmania, Human Cells and Mouse Testis Zemfira N. Karamysheva2, Elena B. Tikhonova1, Petar N. Grozdanov1, James C. Huffman2,3, Kristen R. Baca1,3, Alexander Karamyshev1, R. Brian Denison1, Clinton C. MacDonald1, Kai Zhang2, Andrey L. Karamyshev1 1Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 2Department of Biological Sciences, Texas Tech University, 3CISER (Center for the Integration of STEM Education & Research), Texas Tech University The overall goal of polysome profiling technique is analysis of translational activity of individual mRNAs or transcriptome mRNAs during protein synthesis. The method is important for studies of protein synthesis regulation, translation activation and repression in health and multiple human diseases.
Reaction Kinetics and Combustion Dynamics of I4O9 and Aluminum Mixtures Dylan K. Smith1, Michelle L. Pantoya1, Jeffrey S. Parkey2, Mehmet Kesmez2 1Department of Mechanical Engineering, Texas Tech University, 2Lynntech Inc. A protocol for measuring flame speeds of a reactive mixture composed of tetraiodine nonoxide (I4O9) and aluminum (Al) is presented. A method for resolving reaction kinetics using differential scanning calorimetry (DSC) is also presented. It was found that I4O9 is 150% more reactive than other iodine(V) oxides.
Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture Graham Bailes1, Margaret Lind1, Andrew Ely1, Marianne Powell2, Jennifer Moore-Kucera3, Carol Miles2, Debra Inglis2, Marion Brodhagen1 1Biology Department, Western Washington University, 2Washington State University Northwestern Research and Extension Center, 3Department of Plant and Soil Science, Texas Tech University Plastic films labeled "biodegradable" are commercially available for agricultural use as mulches. Tillage represents an attractive disposal method, but degradation under field conditions is poorly understood. The purpose of this study was to develop methods for isolating native soil fungi and bacteria that colonize plastic mulch films after field burial.