University of Texas at Tyler 1 article published in JoVE Immunology and Infection Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture Anna M. Biesbrock1, Christopher M. Powell1, Wayne B. Hunter2, Blake R. Bextine1 1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR.
