UT Health Science Center San Antonio 2 articles published in JoVE Medicine Evaluation of Capnography Sampling Line Compatibility and Accuracy when Used with a Portable Capnography Monitor Ruben D. Restrepo1, Ido Karpenkop2, Katherine E. Liu3 1Division of Respiratory Care, UT Health Science Center at San Antonio, 2Research and Development, OEM Engineering, Patient Monitoring, Medtronic, Jerusalem, 3Minimally Invasive Therapies Group, Scientific Communications, Medtronic, Minneapolis The goal of this study was to evaluate the accuracy of capnography sampling lines used in conjunction with a portable bedside capnography monitor. Sampling lines from 7 manufacturers were evaluated for tensile strength, rise time, and ETCO2 accuracy as a function of respiratory rate or supplemental oxygen flow rate. Biology Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A) Yohei Niikura*1, Lei Fang*2, Risa Kitagawa*3, Peizhao Li1, Yao Xi1, Ju You1, Yan Guo2, Katsumi Kitagawa3 1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio CENP-A ubiquitylation is an important requirement for CENP-A deposition at the centromere, inherited through dimerization between cell division, and indispensable to cell viability. Here we describe mass spectrometry analysis to identify ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A) protein.