2 articles published in JoVE
Bacterial Immobilization for Imaging by Atomic Force Microscopy David P. Allison1,2, Claretta J. Sullivan3, Ninell Pollas Mortensen1,2, Scott T. Retterer1,4, Mitchel Doktycz1,4 1Biological and Nanoscale Systems Group, Biosciences Division, Oak Ridge National Laboratory, 2Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 3Department of Surgery, Eastern Virginia Medical School, 4Center for Nanophase Materials Sciences Division, Oak Ridge National Laboratory Live Gram-negative and Gram-positive bacteria can be immobilized on gelatin-coated mica and imaged in liquid using Atomic Force Microscopy (AFM).
Flow Cytometry Analysis of Immune Cells Within Murine Aortas Matthew J. Butcher1, Margo Herre1, Klaus Ley2, Elena Galkina1 1Deptartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 2Division of Inflammation Biology, LaJolla Institute for Allergy and Immunology This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.