2 articles published in JoVE
Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles Shanmugavel Madasamy1, David Liu1, Jason Lundry1, Benjamin Alderete2, Raymond Kong2, J. Paul Robinson3, Alan H.B. Wu1,4, Edward P. Amento5 1Plaxgen Inc, 2Millipore Sigma, 3Cytometry Laboratories, Purdue University, 4San Francisco General Hospital, 2M16 Clinical Chemistry, University of California, San Francisco, 5Molecular Medicine Research Institute The objective of this study was to evaluate in vitro lipid-lowering drug effects in modulating the morphology of cholesterol particles. Comparison of lipid-lowering drugs revealed variations in their effect in modulating the morphological features of cholesterol particles.
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols Gloria Figueroa1, Tiyash Parira1, Alejandra Laverde1, Gianna Casteleiro1, Amal El-Mabhouh2, Madhavan Nair1, Marisela Agudelo1 1Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, 2Millipore Sigma This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.