USDA-ARS Vegetable Crops Research Unit 1 article published in JoVE Environment In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors Prarthana S. Dharampal1, Caitlin M. Carlson2, Luis Diaz-Garcia3,4, Shawn A. Steffan1,5 1Department of Entomology, University of Wisconsin-Madison, 2Department of Bacteriology, University of Wisconsin-Madison, 3Department of Horticulture, University of Wisconsin-Madison, 4Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias, 5USDA-ARS, Vegetable Crop Research Unit Fungicide sprays on flowering plants may expose solitary bees to high concentrations of pollen-borne fungicide residues. Using laboratory-based experiments involving in vitro-reared bee larvae, this study investigates the interactive effects of consuming fungicide-treated pollen derived from host and non-host plants.