2 articles published in JoVE
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads Nidhi Nath1, Becky Godat1, Marjeta Urh1 1Promega Corporation The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.
Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology Danette L. Daniels1, Jacqui Méndez1, Hélène Benink1, Andrew Niles1, Nancy Murphy1, Michael Ford2, Richard Jones2, Ravi Amunugama2, David Allen2, Marjeta Urh1 1Promega Corporation, 2MS Bioworks LLC HaloTag technology is a multifunctional technology which has shown significant success in isolation of both small and large protein complexes from mammalian cells. Here we highlight the advantages of this technology compared to existing alternatives and demonstrate its utility to study numerous aspects of protein function inside eukaryotic cells.