Marshall University Joan C. Edwards School of Medicine 3 articles published in JoVE Immunology and Infection Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate Roy Al Ahmar*1, Brandon D. Kirby*2, Hongwei D. Yu1,2,3 1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, 2Progenesis Technologies LLC, Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine, Marshall University Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA. Immunology and Infection Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection Meagan E. Valentine1, Brandon D. Kirby1, Hongwei D. Yu1,2 1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics, Joan C. Edwards School of Medicine at Marshall University Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications. Biology A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy Xiaoliang Wang*1, Muhammad A. Chaudhry*2, Ying Nie2, Zijian Xie1, Joseph I. Shapiro2, Jiang Liu1,2 1Marshall Institute for Interdisciplinary Research (MIIR), Marshall University, 2Department of Biomedical Sciences, Marshall University Joan C. Edwards School of Medicine This manuscript provides a detailed two-step surgical procedure to perform mouse 5/6th partial nephrectomy (PNx) with pole ligation. Four weeks after surgery, in comparison with sham-operated mice, the PNx mice developed impaired renal function, anemia, cardiac hypertrophy, cardiac fibrosis, and decreased heart systolic and diastolic function.
