Centers for Disease Control and Prevention, Morgantown 1 article published in JoVE Environment Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA Angela R. Lemons1, William G. Lindsley1, Brett J. Green1 1Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment methods.
