Progenesis Technologies, LLC 1 article published in JoVE Immunology and Infection Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection Meagan E. Valentine1, Brandon D. Kirby1, Hongwei D. Yu1,2 1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics, Joan C. Edwards School of Medicine at Marshall University Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications.
