3 articles published in JoVE
Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons Zhenyu Liu1, Katelynne B. Donnelly1, Kara G. Pratt1 1Department of Zoology and Physiology and Program in Neuroscience, University of Wyoming In this paper, we discuss three brain preparations used for whole cell patch clamp recording to study the retinotectal circuit of Xenopus laevis tadpoles. Each preparation, with its own specific advantages, contributes to the experimental tractability of the Xenopus tadpole as a model to study neural circuit function.
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material Yo Ching Cheng*1, Samia Hannaoui*1, Theodore Ralph John2, Sandor Dudas3, Stefanie Czub3, Sabine Gilch1 1Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine, University of Calgary, 2Dept. of Molecular Biology, University of Wyoming, 3Canadian Food Inspection Agency, Lethbridge Laboratories Here, we present a protocol to describe a simple, fast and efficient prion amplification technique, the real-time quaking-induced conversion (RT-QuIC) method.
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads Padmamalini Baskaran1, Baskaran Thyagarajan1 1School of Pharmacy, University of Wyoming This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.