Universidad de Buenos Aires, CONICET 1 article published in JoVE Biology Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence Felipe J. Renna1, Malena Herrera Lopez1, Maria Manifava2, Nicholas T. Ktistakis2, Maria I. Vaccaro1 1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL), Universidad de Buenos Aires, CONICET, 2Signalling Programme, The Babraham Institute The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.
