Sao Lucas Cell Therapy Group 1 article published in JoVE Medicine Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation Leniza Pola-Silva1,2, Fabio Xerfan Nahas3, Flavia Nascimento2, Tatiana Rabelo Santos2, Andrea Moraes Malinverni1, Adelson Alves2, Lydia Masako Ferreira3, Maria Isabel Melaragno1 1Genetics Division, Universidade Federal de São Paulo (UNIFESP), 2TechLife Biotech, São Lucas Cell Therapy Group, 3Division of Plastic Surgery, Universidade Federal de São Paulo (UNIFESP) The present protocol describes an improved methodology for ADSC isolation resulting in a tremendous cellular yield with time gain compared to the literature. This study also provides a straightforward method for obtaining a relatively large number of viable cells after long-term cryopreservation.
