Federal University of Santa Maria 1 article published in JoVE Biochemistry Analyzing Mitochondrial Function in a Drosophila melanogaster PINK1B9-Null Mutant Using High-resolution Respirometry Paula Michelotti*1,2, Tâmie Duarte*1,2, Cristiane L. Dalla Corte1,2 1Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences, Federal University of Santa Maria, 2Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria Here we present a high-resolution respirometry protocol to analyze bioenergetics in PINK1B9-null mutant fruit flies. The method uses the Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocol.
