2 articles published in JoVE
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment Talita Miguel Marin1, Nathalia de Carvalho Indolfo4, Silvana Aparecida Rocco1, Murilo de Carvalho1,2, Marilia Meira Dias1, Graziele Izalina Vasconcelos Bento1, Leandro Oliveira Bortot1, Desirée Cigaran Schuck3, Márcio Lorencini3, Eduardo Pagani1 1Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), 2Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 3Grupo Boticário, 4Institute of Biology, University of Campinas We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.
A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions Carolina C. Monte-Bello*1,2, Elias F. Araujo*1,3, Marina C.M. Martins1, Valeria Mafra4, Viviane C.H. da Silva1,2, Viviane Celente1, Camila Caldana1,5 1Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in energy and materials (CNPEM), 2University of Campinas (UNICAMP), 3University of Viçosa (UFV), 4CTBE, CNPEM, 5Brazilian Bioethanol Science and Technology Laboratory (CTBE/CNPEM), Max Planck Partner Group A simple, versatile, and low-cost in vitro hydroponic system was successfully optimized, enabling large-scale experiments under sterile conditions. This system facilitates the application of chemicals in a solution and their efficient absorption by roots for molecular, biochemical, and physiological studies.