Universidade Estadual Paulista 1 article published in JoVE Biology Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects Camila B. Giuliano1,2, Rongjing Zhang1, Laurence G. Wilson1 1The Rowland Institute, Harvard University, 2Faculdade de Ciências e Letras de Assis, Universidade Estadual Paulista The three-dimensional locations of weakly-scattering objects can be uniquely identified using digital inline holographic microscopy (DIHM), which involves a minor modification to a standard microscope. Our software uses a simple imaging heuristic coupled with Rayleigh-Sommerfeld back-propagation to yield the three-dimensional position and geometry of a microscopic phase object.
