Hospital Sirio-Libanes 2 articles published in JoVE Neuroscience Positron Emission Tomography Imaging for In Vivo Measuring of Myelin Content in the Lysolecithin Rat Model of Multiple Sclerosis Daniele de Paula Faria1, Caroline Cristiano Real1, Larissa Estessi de Souza1, Alexandre Teles Garcez2, Fabio Luis Navarro Marques1, Carlos Alberto Buchpiguel1 1Laboratory of Nuclear Medicine (LIM-43), Departamento de Radiologia e Oncologia, Faculdade de Medicina, Universidade de Sao Paulo, 2Nuclear Medicine Division, Hospital Sirio-Libanes This protocol has the aim of monitoring in vivo myelin changes (demyelination and remyelination) by positron emission tomography (PET) imaging in an animal model of multiple sclerosis. Cancer Research Quantification of Tumor Cell Adhesion in Lymph Node Cryosections Elisa Helena Farias Jandrey1, Mayra Akemi Kuroki1, Anamaria Aranha Camargo1, Erico Tosoni Costa1 1Molecular Oncology Center, Hospital Sírio-Libanês Here, we describe a simple and inexpensive method that allows the quantification of adhesive tumor cells to lymph node (LN) cryosections. LN-adherent tumor cells are readily identified by light microscopy and confirmed by a fluorescence-based method, giving an adhesion index that reveals the tumor cell-binding affinity to LN parenchyma.
