3 articles published in JoVE
Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models Federico Perdomo-Celis1,2, Sandra Medina-Moreno1, Alonso Heredia1, Harry Davis1, Joseph Bryant1, Juan Carlos Zapata1 1Institute of Human Virology, School of Medicine, University of Maryland, 2Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia Described here are three experimental approaches for studying the dynamics of HIV infection in humanized mice. The first permits the study of chronic infection events, whereas the two latter allows for the study of acute events after primary infection or viral reactivation.
Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles Sandra L. Arias1, Akshath R. Shetty2, Angana Senpan3, Mónica Echeverry-Rendón4, Lisa M. Reece5,6, Jean Paul Allain1,2,3,7 1Department of Bioengineering, University of Illinois at Urbana-Champaign, 2Department of Nuclear, Plasma and Radiological Engineering, University of Illinois at Urbana-Champaign, 3Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, 4Program of Study and Control of Tropical Diseases (PECET), University of Antioquia, 5Sealy Center for Vaccine Development, University of Texas Medical Branch, 6WHO Collaborating Center for Vaccine Research, Evaluation and Training on Emerging Infectious Diseases, University of Texas Medical Branch, 7Beckman Institute, University of Illinois at Urbana-Champaign Here, we present a protocol to make a bacterial nanocellulose (BNC) magnetic for applications in damaged blood vessel reconstruction. The BNC was synthesized by G. xylinus strain. On the other hand, magnetization of the BNC was realized through in situ precipitation of Fe2+ and Fe3+ ferrous ions inside the BNC mesh.
Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs Sara M. Robledo1, Lina M. Carrillo1,2, Alejandro Daza1, Adriana M. Restrepo1, Diana L. Muñoz1, Jairo Tobón1, Javier D. Murillo1, Anderson López1, Carolina Ríos1, Carol V. Mesa1, Yulieth A. Upegui1, Alejandro Valencia-Tobón1, Karina Mondragón-Shem1, Berardo RodrÍguez2, Iván D. Vélez1 1Program for the Study and Control of Tropical Diseases -PECET-School of Medicine, University of Antioquia, 2School of Agrarian Sciences, University of Antioquia Optimization of the experimental hamster model for cutaneous leishmaniasis by intradermal injection of Leishmania promastigotes at the dorsal skin. This approach is useful during inoculation, follow-up, characterization of lesions, application of treatments and obtaining of clinical samples. Locomotion, search for food and water, play and social activities are preserved.