National University of Colombia 2 articles published in JoVE Neuroscience Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells Carolina Galeano-Naranjo1,2, A. Catalina Veléz-Ortega1, Gregory I Frolenkov1 1Department of Physiology, College of Medicine, University of Kentucky, 2Universidad Nacional de Colombia Here we present a protocol for the Hopping Probe Ion Conductance Microscopy (HPICM), a non-contact scanning probe technique that allows nanoscale imaging of stereocilia bundles in live auditory hair cells. Behavior Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats César Acevedo-Triana1,6, Javier L. Rico2, Leonardo A. Ortega2, Melissa Andrea N. Cardenas3, Fernando P. Cardenas3, Manuel J. Rojas4, Juan Carlos Forigua-Vargas2, Julián Cifuentes2, Camilo Hurtado-Parrado2,5 1School of Psychology, Universidad Pedagógica y Tecnológica de Colombia, 2Animal Behavior Laboratory, Fundación Universitaria Konrad Lorenz, 3Department of Psychology, Universidad de Los Andes, 4School of Veterinary Medicine, Animal Health Department, Universidad Nacional de Colombia, 5Department of Psychology, Troy University, 6Department of Neurobiology, University of Alabama at Birmingham We describe an extended fear-conditioning protocol that produces overtraining and fear incubation in rats. This protocol entails a single training session with 25 tone-shock pairings (i.e., overtraining) and a comparison of conditioned freezing responses during context and cue tests 48 h (short-term) and 6 weeks (long-term) after training.
