3 articles published in JoVE
Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions Carmen Bolatto*1, Silvia Olivera-Bravo*2, Sofía Cerri1,2 1Developmental Biology Laboratory, Histology and Embryology Department, Faculty of Medicine, Universidad de la República, 2Cell and Molecular Neurobiology Laboratory, Clemente Estable Biology Research Institute (IIBCE), Ministerio de Educación y Cultura The ability to accurately detect neuromuscular junction components is crucial in evaluating modifications in its architecture because of pathological or developmental processes. Here we present a complete description of a straightforward method to obtain high-quality images of whole-mount neuromuscular junctions that can be used to perform quantitative measurements.
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos Daniel Prieto1, Gonzalo Aparicio1,2, Matías Machado1, Flavio R. Zolessi1,2 1Institut Pasteur de Montevideo, 2Sección Biología Celular, Facultad de Ciencias, Universidad de la República A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.
Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections Natalia Muñoz-Wolf1,2, Analía Rial1, José M. Saavedra1, José A. Chabalgoity1 1Departamento de Desarrollo Biotecnológico, Universidad de la República, 2Present Affiliation: Trinity Biomedical Science Institute, Trinity College Dublin The present work illustrates the convenience of using sublingual immunotherapy to boost the innate immune response in the lungs and confer protection against acute pneumococcal pneumonia in mouse.