Universidad de la Republica

5 articles published in JoVE

• Developmental Biology

  
  Polysome Purification from Soybean Symbiotic Nodules María Martha Sainz¹, Carla Valeria Filippi¹, Guillermo Eastman^2,3, Mariana Sotelo-Silveira¹, C. Mauro Martinez¹, Omar Borsani¹, José Sotelo-Silveira^{2,4 1}Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, ²Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, ³Department of Biology, University of Virginia, ⁴Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels.

• Developmental Biology

  
  Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots Ines Rauschert*², Juan Claudio Benech*², Martha Sainz¹, Omar Borsani¹, Mariana Sotelo-Silveira^{1 1}Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República (UdelaR), ²Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE) The atomic force microscopy indentation protocol offers the possibility to dissect the role of the physical properties of the cell wall of a particular cell of a tissue or organ during normal or constrained growth (i.e., under water deficit).

• Neuroscience

  
  Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions Carmen Bolatto*¹, Silvia Olivera-Bravo*², Sofía Cerri^{1,2 1}Developmental Biology Laboratory, Histology and Embryology Department, Faculty of Medicine, Universidad de la República, ²Cell and Molecular Neurobiology Laboratory, Clemente Estable Biology Research Institute (IIBCE), Ministerio de Educación y Cultura The ability to accurately detect neuromuscular junction components is crucial in evaluating modifications in its architecture because of pathological or developmental processes. Here we present a complete description of a straightforward method to obtain high-quality images of whole-mount neuromuscular junctions that can be used to perform quantitative measurements.

• Developmental Biology

  
  Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos Daniel Prieto¹, Gonzalo Aparicio^1,2, Matías Machado¹, Flavio R. Zolessi^{1,2 1}Institut Pasteur de Montevideo, ²Sección Biología Celular, Facultad de Ciencias, Universidad de la República A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.

• Medicine

  
  Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections Natalia Muñoz-Wolf^1,2, Analía Rial¹, José M. Saavedra¹, José A. Chabalgoity^{1 1}Departamento de Desarrollo Biotecnológico, Universidad de la República, ²Present Affiliation: Trinity Biomedical Science Institute, Trinity College Dublin The present work illustrates the convenience of using sublingual immunotherapy to boost the innate immune response in the lungs and confer protection against acute pneumococcal pneumonia in mouse.