Yunnan Academy of Tobacco Agricultural Sciences 1 article published in JoVE Biology Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance Yutong Liu1, Mingming Sun1, Zipeng Jiang1, Xinfang Wang2, Bingguang Xiao3, Aiguo Yang1, He Meng1, Lirui Cheng1 1Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, 2Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, 3Yunnan Academy of Tobacco Agricultural Sciences Here, a protocol is presented for the efficient and accurate screening of tobacco genotypes for Phytophthora nicotianae resistance in seedlings. This is a practical approach for precision breeding, as well as molecular mechanism research.