Anhui Province Engineering Laboratory of Occupational Health and Safety 2 articles published in JoVE Medicine Using Nicotine in a Silica-Exposed Mouse Model to Promote Lung Epithelial-Mesenchymal Transition Haoming Chen*1,2,3,4, Bing Li*1,2,3,4, Hangbing Cao1,2,3,4, Yehong Zhao1,2,3,4, Yuanjie Zou1,2,3,4, Wenyang Wang1,2,3,4, Min Mu1,2,3,4, Xinrong Tao1,2,3,4 1Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, 2Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, 3Anhui Province Engineering Laboratory of Occupational Health and Safety, 4School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology This study describes a mouse model to study the synergistic effect of nicotine on the progression of pulmonary fibrosis in experimental silicosis mice. The dual-exposure mouse model simulates the pathological progression in the lung after simultaneous exposure to nicotine and silica. The methods described are simple and highly reproducible. Medicine A Silicosis Mouse Model Established by Repeated Inhalation of Crystalline Silica Dust Hangbing Cao*1,2,3,4, Bing Li*1,2,3,4, Haoming Chen1,3,4, Yehong Zhao1,4, Yuanjie Zou1,4, Yang Liu1,4, Min Mu1,2,3,4, Xinrong Tao1,2,3,4 1Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, 2Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, 3Anhui Province Engineering Laboratory of Occupational Health and Safety, 4School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology This protocol describes a method for establishing a mouse model of silicosis through repeated exposure to silica suspensions via a nasal drip. This model can efficiently, conveniently, and flexibly mimic the pathological process of human silicosis with high repeatability and economy.
