Gifu University 2 articles published in JoVE Biochemistry Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology Sayuri L. Higashi1,2, Kazuya Yagyu1,2, Haruna Nagase1,2, Craig S. Pearson1, Herbert M. Geller1, Yasuhiro Katagiri1 1Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 2United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University An ultra-high-speed western blotting technique is developed by improving the kinetics of antigen-antibody binding through cyclic draining and replenishing (CDR) technology in conjunction with an immunoreaction enhancing agent. Environment Evaluation of the Productivity of Social Wasp Colonies (Vespinae) and an Introduction to the Traditional Japanese Vespula Wasp Hunting Technique Tatsuya Saga1,2 1Tajimi Senior High School, 2Faculty of Applied Biological Science, Gifu University This methodological paper evaluates the productivity of a social wasp colony by examining the number of meconia per 100 cells of comb, to estimate the total number of adults the wasps produced. The associated video describes how to search for Vespula wasp nests, a method developed by amateur wasp chasers.