Kyoto Institute of Technology 2 articles published in JoVE Genetics Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains Kaori Nishimura*1, Miho Asaoka*2, Yurina Sakamaki*2, Tatsuya Fukumoto*3,4, Daisuke Tanaka3, Satoru Kobayashi2, Toshiyuki Takano-Shimizu-Kouno1 1KYOTO Drosophila Stock Center, Kyoto Institute of Technology, 2Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 3Research Center of Genetic Resources, National Agriculture and Food Research Organization, 4Shizuoka Prefectural Ogasa High School A long-term preservation method for Drosophila strains as an alternative to the frequent transfer of adult flies to fresh food vials is highly desirable. This protocol describes the cryopreservation of Drosophila primordial germ cells and strain revival via their transplantation to agametic host embryos. Biology Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate Robert M. Cooper*1, Taishi Tonooka*1,2, Andriy Didovyk*1,3, Jeff Hasty1,4,5 1Biocircuits Institute, University of California, San Diego, 2Present address, Kyoto Institute of Technology, 3Present address, Vertex Pharmaceuticals, 4Department of Bioengineering, University of California, San Diego, 5Molecular Biology Section, University of California, San Diego This protocol describes a rapid and simple method to produce bacterial lysate for cell-free gene expression, using an engineered strain of Escherichia coli and requiring only standard laboratory equipment.
