CSIRO (Commonwealth Scientific and Industrial Research Organisation) View Institution's Website 4 articles published in JoVE Engineering Recombination Dynamics in Thin-film Photovoltaic Materials via Time-resolved Microwave Conductivity Joanna A. Guse1,2, Timothy W. Jones3, Andrew Danos4, Dane R. McCamey1,2 1ARC Centre of Excellence in Exciton Science, 2School of Physics, University of New South Wales, 3CSIRO, CSIRO Energy Centre, 4School of Chemistry, University of New South Wales A Time Resolved Microwave Conductivity technique for investigating direct and trap-mediated recombination dynamics and determining carrier mobilities of thin film semiconductors is presented here. Biology Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2 Juan Carlos Polanco1, Bei Wang1, Qi Zhou1, Hun Chy1, Carmel O'Brien1, Andrew L. Laslett1 1Materials Science and Engineering, CSIRO We describe the use of the monoclonal antibodies TG30 (CD9) and GCTM-2 for the combined detection of cell surface antigens via fluorescence activated cell sorting (FACS) for the identification and enrichment of live human embryonic stem cells (hESC) using positive selection and also the use of negative selection to purge hESCs from a mixed cell population. Biology Glycan Profiling of Plant Cell Wall Polymers using Microarrays Isabel E. Moller1,2, Filomena A. Pettolino3, Charlie Hart1, Edwin R. Lampugnani1, William G.T. Willats4, Antony Bacic1,2 1Australian Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, 2Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3CSIRO Plant Industry, Black Mountain Laboratories, 4Department of Plant Biology and Biotechnology, University of Copenhagen A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts. Bioengineering Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming Wah Chin Boon1, Karolina Petkovic-Duran2, Yonggang Zhu2, Richard Manasseh3, Malcolm K. Horne1, Tim D. Aumann1 1Florey Neuroscience Institutes and Centre for Neuroscience, University of Melbourne, 2Fluid Dynamics Group, CSIRO Materials Science and Engineering, 3Swinburne University of Technology, Faculty of Engineering and Industrial Sciences We describe a novel method for increasing cDNA yield from single-cell quantities of mRNA in otherwise standard laboratory reverse transcription reactions. The novelty resides in the use of a micromixer, which utilizes the phenomenon of acoustic microstreaming, to mix fluids at microliter scales more effectively than shaking, vortexing or trituration.