Barcelona Supercomputing Center 1 article published in JoVE Genetics An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations Llorenç Rovirosa*1, Laureano Tomás-Daza*1,2, Blanca Urmeneta1, Alfonso Valencia2,3, Biola M. Javierre1,4 1Josep Carreras Leukaemia Research Institute, 2Barcelona Supercomputing Center, 3Catalan Institution for Research and Advanced Studies (ICREA), 4Institute for Health Science Research Germans Trias i Pujol Gene expression is regulated by interactions of gene promoters with distal regulatory elements. Here, we descirbe how low input Capture Hi-C (liCHi-C) allows the identification of these interactions in rare cell types, which were previously unmeasurable.
