The Pirbright Institute 1 article published in JoVE Immunology and Infection An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis Katherine L. Dulwich*1, Amin S. Asfor*1, Alice G. Gray1, Venugopal Nair1, Andrew J. Broadbent1 1The Pirbright Institute Here we describe the isolation of chicken primary bursal cells from the bursa of Fabricius, the culture and infection of the cells with infectious bursal disease virus, and the quantification of viral replication.