Women's College Research Institute 1 article published in JoVE Immunology and Infection Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses Jeeyoon Jennifer Ahn*1, Thirumahal Selvanantham*1, Monan Angela Zhang*1, Thierry Mallevaey1, Shannon E. Dunn1,2,3 1Department of Immunology, University of Toronto, 2Toronto General Research Institute, University Health Network, 3 This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.