Angiocrine Biosciences, Inc. 1 article published in JoVE Bioengineering Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery Limor Man1, Laura Park1, Richard Bodine1, Michael Ginsberg2, Nikica Zaninovic3, Glenn Schattman1, Robert E. Schwartz4, Zev Rosenwaks1,3, Daylon James1,3 1Center for Reproductive Medicine and Infertility, Weill Cornell Medical College, 2Angiocrine Biosciences, Inc., 3Tri-Institutional Stem Cell Derivation Laboratory, Weill Cornell Medical College, 4Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medical College For some patients, the only option for fertility preservation is cryopreservation of ovarian tissue. Unfortunately, delayed revascularization undermines follicular viability. Here, we present a protocol to co-transplant human ovarian tissue with endothelial cells for utilization as a cell-based strategy combining accelerated perfusion with a direct paracrine delivery of bioactive molecules.