University of Nebraska College of Medicine 1 article published in JoVE Medicine Microfluidic Model of Necrotizing Enterocolitis Incorporating Human Neonatal Intestinal Enteroids and a Dysbiotic Microbiome Lauren C. Frazer1, Yukihiro Yamaguchi1, Corey M. Jania1, Wyatt E. Lanik2, Qingqing Gong3, Dhirendra K. Singh1, Stephen Mackay1, Natalia S. Akopyants1, Misty Good1 1Division of Neonatal-Perinatal Medicine, Department of Pediatrics, University of North Carolina at Chapel Hill, 2University of Nebraska College of Medicine, 3Department of Surgery, Washington University School of Medicine This protocol describes an in vitro model of necrotizing enterocolitis (NEC), which can be used for mechanistic studies into disease pathogenesis. It features a microfluidic chip seeded with intestinal enteroids derived from the human neonatal intestine, endothelial cells, and the intestinal microbiome of a neonate with severe NEC.