IBM 1 article published in JoVE Biology Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry Diamantis G. Konstantinidis1, Suvarnamala Pushkaran1, Katie Giger1, Stefanos Manganaris2, Yi Zheng1, Theodosia A. Kalfa1 1Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 2IBM The present protocol describes a novel method of identifying a population of enucleating orthochromatic erythroblasts by multi-spectral imaging flow cytometry, providing a visualization of the erythroblast enucleation process.