KCAS Bioanalytical and Biomarker Services 1 article published in JoVE Biology A Validatable Droplet Digital Polymerase Chain Reaction Assay for the Detection of Adeno-Associated Viral Vectors in Bioshedding Studies of Tears Bryanna Longacre1, Amanda Souaysene1, Carrie A. Vyhlidal1, Matthew R. Pennington1 1Cell and Gene Therapy, KCAS Bioanalytical and Biomarker Services Here, we present a protocol for the development and validation of good laboratory practices in the compliant detection of adeno-associated viral vectors in human tears by droplet digital polymerase chain reaction in support of clinical development of gene therapy vectors.
