Federal University of Rio De Janeiro 7 articles published in JoVE Biology Establishing a Diaphyseal Femur Fracture Model in Mice Bianca Braga Frade*1, Leonardo Dias da Cunha Muller*2, Danielle Cabral Bonfim3 1Postgraduate Program in Biological Sciences – Biophysics, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, 2Postgraduate Program in Surgical Sciences, Faculty of Medicine, Federal University of Rio de Janeiro, 3Institute of Biomedical Sciences, Federal University of Rio de Janeiro This protocol describes a surgical procedure for the establishment of a diaphyseal fracture in the femur of mice, which is stabilized with an intramedullary wire, for fracture healing studies. Bioengineering Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting Gabriela S. Kronemberger1,2,3, Guilherme A. S. C. Miranda1,2,4, Taisnara I. G. Silva1,2,4, Rosângela M. Gonçalves1,2, José M. Granjeiro2,3,4,5, Leandra S. Baptista1,2,3,4 1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense Here, we describe the large-scale production of adipose-derived stromal/stem cell (ASC) spheroids using an automated pipetting system to seed the cell suspension, thus ensuring homogeneity of spheroid size and shape. These ASC spheroids can be used as building blocks for 3D bioprinting approaches. Environment A Field Primer for Monitoring Benthic Ecosystems Using Structure-From-Motion Photogrammetry Ty N. F. Roach1, Shreya Yadav1, Carlo Caruso1, Jenna Dilworth1,2, Catherine M. Foley1, Joshua R. Hancock1, Joel Huckeba1, Ariana S. Huffmyer1, Kira Hughes1, Valerie A. Kahkejian1, Elizabeth M.P. Madin1, Shayle B. Matsuda1, Michael McWilliam1, Spencer Miller1,3, Erika P. Santoro4, Mariana Rocha de Souza1, Damaris Torres-Pullizaa1, Crawford Drury1, Joshua S. Madin1 1Hawai'i Institute of Marine Biology, University of Hawai'i Manoa, 2Rosenstiel School of Marine and Atmospheric Science, University of Miami, 3Hawai'i Pacific University, 4Federal University of Rio de Janeiro We provide a detailed protocol for conducting underwater structure-from-motion photogrammetry surveys to generate 3D models and orthomosaics. Bioengineering Generating a Fractal Microstructure of Laminin-111 to Signal to Cells Camila Hochman-Mendez1, Tatiana Coelho-Sampaio2, Ariel J. Kent3, Jamie L. Inman3, Mina J. Bissell3, Claire Robertson4 1Regenerative Medicine Research, Texas Heart Institute, 2Institute of Biomedical Sciences, Federal University of Rio de Janeiro, 3Biological Systems and Engineering, Lawrence Berkeley National Lab, 4Materials Engineering Division, Lawrence Livermore National Lab We describe three methods to generate Ln1 polymers with fractal properties that signal to cells differently compared to unpolymerized Ln1. Bioengineering Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation Helena D. M. Villela1, Caren L. S. Vilela1, Juliana M. Assis1, Natascha Varona2, Camille Burke2, David A. Coil2, Jonathan A. Eisen2, Raquel S. Peixoto1,2,3 1LEMM, Laboratory of Molecular Microbial Ecology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (UFRJ), 2Genome Center, University of California, Davis, 3IMAM-AquaRio - Rio de Janeiro Aquarium Research Center Pollution affects all biomes. Marine environments have been particularly impacted, especially coral reefs, one of the most sensitive ecosystems on Earth. Bioremediation is the capacity of organisms to degrade contaminants. Here, we describe methodologies to isolate and test microbes presenting bioremediation ability and potential probiotic characteristics for corals. Bioengineering Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins Anna C. N. T. F. Corrêa1, Patricia R. Pereira1, Vânia M. F. Paschoalin1 1Chemistry Institute, Federal University of Rio de Janeiro This study describes classical hydration using the thin lipid film method for nanoliposome preparation followed by nanoparticle characterization. A 47 kDa-hydrophilic and globular protein, tarin, is successfully encapsulated as a strategy to improve stability, avoid fast clearance, and promote controlled release. The method can be adapted to hydrophobic molecules encapsulation. Chemistry CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis Rafael D. Melani*1,2, Henrique S. Seckler*1, Owen S. Skinner1, Luis H. F. Do Vale1,3, Adam D. Catherman1, Pierre C. Havugimana1, Marcelo Valle de Sousa3, Gilberto B. Domont2, Neil L. Kelleher1, Philip D. Compton1 1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.
