Tecnologia e Innovacion (SNI-SENACYT) 1 article published in JoVE Genetics Development of Leishmania Species Strains with Constitutive Expression of eGFP Jafeth Carrasco*1, Jim H. Chang*1, Laura Pineda1, Iliana Quintero1, Rita Giovani2, Carmenza Spadafora1,3, Ricardo Lleonart1,3, Carlos M. Restrepo1,3 1Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), 2Departamento de Medios y Creativo, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), 3Sistema Nacional de Investigación-Secretaría Nacional de Ciencia, Tecnología e Innovación (SNI-SENACYT) Here, we describe the methodology used for generating L. panamensis and L. donovani strains expressing the gene for eGFP as a stable integrated transgene using the pLEXSY system. Transfected parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity in both species were selected for further use in drug screening assays.
