Plant Cell Biology
Plant Cell Biology - Related Videos
12:30
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
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Cited by 25 •
2012
A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.
11:42
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
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Cited by 32 •
2016
We describe a robust gene replacement strategy to genetically manipulate the smut fungus Ustilago maydis. This protocol explains how to generate deletion mutants to investigate infection phenotypes. It can be extended to modify genes in any desired way, e.g., by adding a sequence encoding a fluorescent protein tag.
07:43
Assaying Proteasomal Degradation in a Cell-free System in Plants
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Cited by 26 •
2014
Targeted protein degradation represents a major regulatory mechanism for cell function. It occurs via a conserved ubiquitin-proteasome pathway, which attaches polyubiquitin chains to the target protein that then serve as molecular “tags” for the 26S proteasome. Here, we describe a simple and reliable cell-free assay for proteasomal degradation of proteins.
13:35
Fluorescence Activated Cell Sorting of Plant Protoplasts
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Cited by 83 •
2010
A method for isolating specific cell types from plant material is demonstrated. This technique employs transgenic marker lines expressing fluorescent proteins in particular cell types, cellular dissociation and Fluorescence Activated Cell Sorting. Additionally, a growth setup is established here that facilitates treatment of Arabidopsis thaliana seedlings prior to cell sorting.
08:39
Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
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Cited by 6 •
2017
Plant defensins play an important role in plant defense against pathogens. For effective use of these antifungal peptides as antifungal agents, understanding their modes of action (MOA) is critical. Here, a live-cell imaging method is described to study critical aspects of the MOA of these peptides.
FAQs
These organelles make distinct contributions while operating as part of a coordinated cellular system. Chloroplasts support photosynthesis, vacuoles participate in cellular organization and regulation, and mitochondria contribute to the broader activities required for plant cell function. Studying their coordination helps explain how cells sustain growth, development, environmental responses, and reproduction rather than treating each organelle as an isolated unit.
The cell wall provides structural organization, while the plasma membrane regulates transport and helps mediate signaling with the environment. The cytoskeleton contributes to cell shape and internal organization. Examining these components together clarifies how plant cells maintain their form, control exchanges, communicate with external conditions, and coordinate processes involved in growth and development.
Environmental stress can be examined through the combined behavior of membranes, cell walls, cytoskeletal structures, and organelles. These components influence transport, signaling, cellular shape, and internal coordination as conditions change. Molecular analysis and microscopy allow researchers to investigate how such changes affect plant cell function, providing a cellular perspective on broader plant responses to stress.
Plant cell research commonly combines microscopy, cell culture, genetics, and molecular analysis. Microscopy examines cellular structures and organization, whereas cell culture provides a controlled setting for studying plant cells. Genetics and molecular approaches investigate how cellular processes are regulated. Using these methods together connects visible cell behavior with underlying biological mechanisms.
Applications arise when researchers connect cellular processes with traits important to agriculture. Studies of photosynthesis, cell division, differentiation, disease resistance, and stress responses can inform crop improvement and biotechnology. This cellular knowledge also supports efforts toward sustainable agriculture by helping researchers understand how plants function, develop, and interact with challenging environmental conditions.
The field can link cellular mechanisms to major biological outcomes, including plant growth, development, reproduction, and responses to stimuli. It also provides a basis for investigating disease resistance and improving biotechnology strategies. Because these outcomes depend on coordinated cellular activity, research integrates structures, processes, and molecular evidence to interpret plant behavior across multiple levels.