JoVE Journal
Biology
Biology
È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.
Capitoli
Riepilogo
In this protocol we demonstrate the expression, solubilization, and purification of a recombinantly expressed membrane protein, MexB, as a soluble protein detergent complex. MexB is a multidrug resistance membrane transporter from the opportunistic bacterial pathogen Pseudomonas aeruginosa.