Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Tae-Young Choi; Mehwish Khaliq; Sungjin Ko; Juhoon So; Donghun Shin

doi:10.3791/52785

Rivista

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Biologia dello sviluppo

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Ablazione specifiche Hepatocyte in Zebrafish allo Studio biliare-driven Liver Regeneration

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Ablazione specifiche Hepatocyte in Zebrafish allo Studio biliare-driven Liver Regeneration

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8,539 Views

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08:14 min

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May 20, 2015

DOI:

10.3791/52785-v

DOI:

10.3791/52785-v

•

08:14 min

May 20, 2015

•

5 Views

Tae-Young Choi¹, Mehwish Khaliq¹, Sungjin Ko¹, Juhoon So¹, Donghun Shin¹

¹Department of Developmental Biology, McGowan Institute for Regenerative Medicine,University of Pittsburgh

Summary

To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.