Imaging Subcellular Structures in the Living Zebrafish Embryo

Peter Engerer; Gabriela Plucinska; Rachel Thong; Laura Trov&ograve;; Dominik Paquet; Leanne Godinho

doi:10.3791/53456

Rivista

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Biologia dello sviluppo

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Imaging strutture subcellulari nell'embrione Living Zebrafish

Imaging Subcellular Structures in the Living Zebrafish Embryo

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging strutture subcellulari nell'embrione Living Zebrafish

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11,519 Views

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11:19 min

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April 02, 2016

DOI:

10.3791/53456-v

DOI:

10.3791/53456-v

•

11:19 min

April 02, 2016

•

4 Views

Peter Engerer¹, Gabriela Plucinska^1,2, Rachel Thong^1,3, Laura Trovò¹, Dominik Paquet^4,5,6, Leanne Godinho¹

¹Institute of Neuronal Cell Biology,Technische Universität München, ²Cell Biology, Department of Biology, Faculty of Science,Utrecht University, ³Faculty of Biology,Ludwig-Maximilians-Universität-München, ⁴Adolf-Butenandt-Institute, Biochemistry,Ludwig-Maximilians-Universität-München, ⁵German Center for Neurodegenerative Diseases, ⁶Laboratory of Brain Development and Repair,The Rockefeller University

Trascrizione

Summary

Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.