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Isolamento e<em> Ex Vivo</em> Cultura di Vδ1<sup> +</sup> CD4<sup> +</sup> Celle γδ T, un Extrathymic αβT-cellule progenitrici
Isolation and <em>Ex Vivo</em> Culture of Vδ1<sup>+</sup>CD4<sup>+</sup>γδ T Cells, an Extrathymic αβT-cell Progenitor
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

Isolamento e<em> Ex Vivo</em> Cultura di Vδ1<sup> +</sup> CD4<sup> +</sup> Celle γδ T, un Extrathymic αβT-cellule progenitrici

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10:33 min

December 07, 2015

DOI:

10:33 min
December 07, 2015

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Summary

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Here, we provide an optimized protocol for the isolation and cloning of the scarce T-cell entity of peripheral Vδ1+CD4+ T cells that is, as we showed recently, an extrathymic αβ T-cell progenitor. This technique allows to quantitatively isolate, clone and efficiently expand these cells in ex vivo culture.

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