Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / High-fat Feeding Paradigm for Larval Zebrafish:…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Biology
High-fat Feeding Paradigm for Larval Zebrafish
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Journal (Biology)
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Oil Red O Staining and Measurement of Lipid Droplets (LDs) in Bovine Hepatocyte Cells (Video) | JoVE
Journal (Biology)
Oil Red O Staining and Measurement of Lipid Droplets (LDs) in Bovine Hepatocyte Cells (Video) | JoVE
 

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake

Article DOI: 10.3791/54735-v • 11:30 min • October 27th, 2016
October 27th, 2016 •
1, 1,2
1Department of Embryology, Carnegie Institution for Science, 2Department of Biology, Johns Hopkins University

Capitoli

Riepilogo
Automatic Translation

Zebrafish are emerging as a valuable model of dietary lipid processing and metabolic disease. Described are protocols of lipid-rich larval feeds, live imaging of dietary fluorescent lipid analogs, and quantification of food intake. These techniques can be applied to a variety of screening, imaging, and hypothesis driven inquiry techniques.

Tags

High-fat Feeding Paradigm Larval Zebrafish Fluorescent Lipid Analog Food Intake Lipid Uptake Dynamics Quantify Dietary Lipid Intake Metabolism Gastrointestinal Biology Fluorescent Lipid Analogs Microscopy Subcellular Lipid Processing Powdered Fluorescent Lipid Analogs Chloroform Amber Glass Tubes Teflon Gasket Freezer Nitrogen Stream Ethanol Embryo Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter