Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Efficient Generation and Editing of Feeder-free…
JoVE Journal
Bioengineering

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Bioengineering
Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
Journal (Bioengineering)
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
Journal (Bioengineering)
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
 

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Article DOI: 10.3791/56260-v • 09:16 min • November 8th, 2017
November 8th, 2017 •
1,2, 3, 1,2,4
1Department of Animal and Avian Sciences, University of Maryland, 2Animal Bioscience and Biotechnology Laboratory, ARS, USDA, 3NIH Stem Cell Unit, Bethesda, National Institutes of Health, 4RenOVAte Biosciences Inc

Capitoli

Riepilogo
Automatic Translation

This protocol describes in detail the generation of footprint-free induced pluripotent stem cells (iPSCs) from human pancreatic cells in feeder-free conditions, followed by editing using CRISPR/Cas9 ribonucleoproteins and characterization of the modified single-cell clones.

Tags

Feeder-free IPSCs Human Pancreatic Cells CRISPR-Cas9 System Footprint-free IPSCs Genome Editing CRISPR-Cas9 Ribonucleoproteins Single Cell Clones Clonal Lines Mosaicism Collagen Coating Prigrow III Medium Transduction Reprogramming Virus Mixture
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter