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流式细胞术对小鼠原发性胰腺导管腺癌原位同种异体 (自体) 免疫分型的研究
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Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
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流式细胞术对小鼠原发性胰腺导管腺癌原位同种异体 (自体) 免疫分型的研究

DOI:
10.3791/57460-v

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08:30 min

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October 09, 2018

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, , , , , , ,
1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs,Peking University

Capitoli

  • 00:04Titolo
  • 00:33Preparation for Tumor Transplantation
  • 01:40Surgical Operation for Orthotopic Implantation
  • 02:58Tumor Tissue Dissociation and Single Cell Preparation
  • 05:01Flow Data Acquisition
  • 06:46Results: Comparison of Orthotopic and Subcutaneous Implantation of Pancreatic Ductal Adenocarcinoma
  • 08:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

免疫分型小鼠原位 PDAC homografts 的实验程序旨在分析肿瘤免疫微环境。肿瘤通过手术原位植入。200–600毫米3的肿瘤被收获和分离, 以制备单细胞悬浮液, 然后使用不同的荧光标记抗体进行多免疫标记物的分析。

Tags

ImmunophenotypingMurinePrimary KPC Pancreatic Ductal AdenocarcinomaFlow CytometryC57 Black 6 MiceTumor VolumeOrthotopic HomograftSyngeneicEuthanasiaTumor DissectionTumor ImplantationTumor Monitoring

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