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Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
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Cancer Research
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JoVE Journal Cancer Research
Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

DOI:
10.3791/57460-v

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08:30 min

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October 09, 2018

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, , , , , , ,
1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs,Peking University

Chapters

  • 00:04Title
  • 00:33Preparation for Tumor Transplantation
  • 01:40Surgical Operation for Orthotopic Implantation
  • 02:58Tumor Tissue Dissociation and Single Cell Preparation
  • 05:01Flow Data Acquisition
  • 06:46Results: Comparison of Orthotopic and Subcutaneous Implantation of Pancreatic Ductal Adenocarcinoma
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

The experimental procedure on the immunophenotyping of murine orthotopic PDAC homografts aims at profiling the tumor immuno-microenvironment. Tumors are orthotopically implanted via surgery. Tumors of 200–600 mm3 in size were harvested and dissociated to prepare single-cell suspensions, followed by multi-immune marker FACS analysis using different fluorescently-labeled antibodies.

Tags

Keywords: ImmunophenotypingMurinePrimary KPC Pancreatic Ductal AdenocarcinomaFlow CytometryC57 Black 6 MiceTumor VolumeOrthotopic HomograftSyngeneicEuthanasiaTumor DissectionTumor ImplantationTumor Monitoring

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