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NKG2D 利甘德表面检测的两种流细胞学方法，以区分干细胞与急性骨髓性白血病中的散装亚群
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Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
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NKG2D 利甘德表面检测的两种流细胞学方法，以区分干细胞与急性骨髓性白血病中的散装亚群

DOI:
10.3791/61803-v

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05:24 min

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February 21, 2021

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, , , , , ,
1Department of Biomedicine,University Hospital Basel and University of Basel, 2Division for Hematology,University Hospital Basel and University of Basel, 3Department of Internal Medicine, Hematology and Oncology,University Hospital Tübingen

Capitoli

  • 00:04Introduction
  • 00:43Biotinylation of the NKG2D Fusion Protein and Thawing of Primary AML Cells
  • 02:00Staining of Primary AML Cells with the Biotinylated NKG2D Fusion Protein
  • 03:24Results: Gating Strategy and Staining Protocol Comparison
  • 04:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们在人类原发性急性骨髓性白血病 （AML） 样本中提出了 NKG2D 配体 （NKG2DL） 检测的两种不同的染色方案。第一种方法基于融合蛋白，能够识别所有已知和潜在的未知配体，而第二种方法依赖于添加多个抗NKG2DL抗体。

Tags

NKG2D LigandsFlow CytometryAcute Myeloid LeukemiaLeukemic Stem CellsCell Surface DetectionBiotinylated NKG2D Fusion ProteinStreptavidin PE

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