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Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
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Cancer Research
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JoVE Journal Cancer Research
Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

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3,948 Views

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05:24 min

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February 21, 2021

DOI:

10.3791/61803-v

DOI:
10.3791/61803-v

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05:24 min
February 21, 2021

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1Department of Biomedicine,University Hospital Basel and University of Basel, 2Division for Hematology,University Hospital Basel and University of Basel, 3Department of Internal Medicine, Hematology and Oncology,University Hospital Tübingen

Summary

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We present two different staining protocols for NKG2D ligand (NKG2DL) detection in human primary acute myeloid leukemia (AML) samples. The first approach is based on a fusion protein, able to recognize all known and potentially yet unknown ligands, while the second protocol relies on the addition of multiple anti-NKG2DL antibodies.

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Landerer, H., Arnone, M., Wieboldt, R., Goersch, E., Stanger, A. M. P., Konantz, M., Lengerke, C. Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia. J. Vis. Exp. (168), e61803, doi:10.3791/61803 (2021).

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