Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Bacteriële expressie en zuivering van humane…
JoVE Journal
Biochemistry

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biochemistry
Bacteriële expressie en zuivering van humane matrix metalloproteinase-3 met behulp van affiniteitschromatografie
A Planarian Motility Assay to Gauge the Biomodulating Properties of Natural Products
Journal (Biochemistry)
A Planarian Motility Assay to Gauge the Biomodulating Properties of Natural Products
An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
Journal (Biochemistry)
An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
 
Click here for the English version

Bacteriële expressie en zuivering van humane matrix metalloproteinase-3 met behulp van affiniteitschromatografie

Article DOI: 10.3791/63263-v • 07:32 min • March 30th, 2022
March 30th, 2022 •
*1, *1, 1
1Department of Chemical & Materials Engineering, University of Nevada, Reno
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

His-tag zuivering, dialyse en activering worden gebruikt om de opbrengst van oplosbare, actieve matrix metalloproteinase-3 katalytische domein eiwitexpressie in bacteriën te verhogen. Eiwitfracties worden geanalyseerd via SDS-PAGE gels.

Tags

Biochemie Matrix metalloproteinase MMP-3cd Bacteriële expressie Oplosbaarheid en hervouwen van menselijke eiwitten in E. coli His-tag affiniteit eiwitzuivering.
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter