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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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February 16, 2014
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概要
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Phage display is a powerful technique to capture proteins or protein moieties that interact with an immobilized molecule of interest. Once a decision of the type of phage cDNA library to create and screen has been made, the protocol described here permits efficient affinity selection leading to identification of interactors.
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Kushwaha, R., Schäfermeyer, K. R., Downie, A. B. A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits. J. Vis. Exp. (84), e50685, doi:10.3791/50685 (2014).
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