DNAメチル化のバイオインフォマティクス分析へのサンプル調製:肥満および関連形質研究の関連戦略
6.3K 閲覧数
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被引用数 9
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14:56 分
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2022年5月6日
DOI :10.3791/62598-v
2022年5月6日
•6.3K 閲覧数
Natália Yumi Noronha*1 , Guilherme da Silva Rodrigues*1 , Marcela Augusta de Souza Pinhel1 , Jean-Baptiste Cazier2,3 , Lígia Moriguchi Watanabe1 , Albert Nobre Menezes4 , Carlos Roberto Bueno5 , Carolina Ferreira Nicoletti1 , Bruno Affonso Parenti de Oliveira1 , Isabelle Mello Schineider6 , Isabella Harumi Yonehara Noma7 , Igor Caetano Dias Alcarás8 , Fernando Barbosa9 , Carla Barbosa Nonino6
1Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, 2Centre for Computational Biology, University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport, University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, 7Faculty of Pharmaceutical Sciences, University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo
* These authors contributed equally
本研究では、マイクロアレイ技術によって得られたDNAメチル化データを管理するワークフローについて説明する。このプロトコルは、サンプル調製からデータ分析までの手順を示しています。すべての手順が詳細に説明されており、ビデオは重要な手順を示しています。
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Chapters in this video
0:03
Introduction
0:43
Day 1: Bisulfite Treatment
1:21
Day 2: Amplification - Methylation Assay, Manual Protocol
2:52
Day 3: Hybridization - Methylation Assay, Manual Protocol
6:39
Day 4: Staining - Methylation Assay, Manual Protocol
10:48
Representative Results
14:34
Conclusion
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