Tracing <em>de novo</em> Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Katherine D. Castro; Lilian Valadares Tose; Matthew Willetts; Melvin A. Park; Marcela Nouzova; Fernando G. Noriega; Francisco Fernandez-Lima

doi:10.3791/65590

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Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

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Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

安定同位体標識 LC-TIMS-TOF MS/MS を用いた de novo 脂質の追跡

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07:12 min

August 23, 2024

DOI: 10.3791/65590-v

Katherine D. Castro¹, Lilian Valadares Tose¹, Matthew Willetts², Melvin A. Park², Marcela Nouzova³, Fernando G. Noriega^4,5, Francisco Fernandez-Lima^1,6

¹Deparment of Chemistry and Biochemistry,Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology,Biology Centre CAS, ⁴Department of Biological Sciences,Florida International University, ⁵Department of Parasitology,University of South Bohemia, ⁶Biomolecular Sciences Institute,Florida International University

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Overview

This research presents a method for screening lipid structures through stable isotope labeling, enhancing quantification in lipidemic samples. The study addresses the gap in standardization of LC-TIMS-TOF MS/MS in lipid investigations.

Key Study Components

Area of Science

Neuroscience
Biochemistry
Mass Spectrometry

Background

Lipidemic samples vary significantly across species.
Standardization in lipid analysis is currently lacking.
LC-TIMS-TOF MS/MS is a key technique for lipid analysis.
Understanding lipid panels can aid in diverse biological studies.

Purpose of Study

To implement specific quantification methods for lipids.
To track biomass remains in lipidemic investigations.
To explore future research in epigenetics and metabolomics.

Methods Used

Stable isotope labeling for lipid quantification.
LC-TIMS-TOF MS/MS calibration procedures.
Use of DDA and DIA methods in mass spectrometry.
Analysis of diverse samples from insects to humans.

Main Results

Developed a method for improved lipid structure screening.
Identified the need for standardization in lipid analysis.
Provided a framework for future research in related fields.
Demonstrated the application of stable isotope labeling.

Conclusions

The method enhances the understanding of lipid diversity.
Standardization is crucial for advancing lipidomic studies.
Future research will expand into epigenetics and metabolomics.

Frequently Asked Questions

What is stable isotope labeling?

Stable isotope labeling is a technique used to track and quantify specific molecules, such as lipids, in biological samples.

Why is standardization important in lipid analysis?

Standardization ensures consistency and reliability in results across different studies and laboratories.

What are LC-TIMS-TOF MS/MS methods?

LC-TIMS-TOF MS/MS refers to liquid chromatography coupled with trapped ion mobility spectrometry and time-of-flight mass spectrometry, used for detailed analysis of complex mixtures.

How does this research impact future studies?

This research lays the groundwork for more precise lipid analysis and opens avenues for exploring epigenetics and metabolomics.

What types of samples were analyzed in this study?

The study analyzed a range of samples from insects to humans to demonstrate the method's applicability.

ここでは、安定同位体標識により、その保持時間、移動度、およびフラグメンテーションを利用した脂質構造をスクリーニングする方法を紹介します。

この研究では、安定同位体標識を使用して、リピドミクスサンプルでより特異的な定量を実施することを目的としています。これにより、脂質パネルや昆虫からヒトまでの非常に多様なサンプルを理解することができます。現在、この分野では、リピドミクス研究におけるLC、TIMS、Top、MS/MSの標準化に関してギャップがあります。

ここでは、この目的のためにバイオマス残骸を追跡する方法を提供します。将来的には、LCチームのスタッフを用いた生体プロセスにおけるエピジェネティクスやメタボロミクスの研究、MS/MS、DDA法、DIA法の研究を行う予定です。

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