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A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

1Department of Radiology, Memorial Sloan Kettering Cancer Center, 2KAUST Catalysis Center, King Abdullah University of Science and Technology, 3Department of Chemistry, Hunter College, and PhD Program in Chemistry, Graduate Center of City University of New York, 4Department of Radiology, Weill Cornell Medical College

JoVE 54694


 Bioengineering

A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents

1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

JoVE 50512


 Biology

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

1Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, 2Current Affiliation is Agency for Defense Development (ADD), Daejeon, Republic of Korea, 3Current Affiliation is KOGAS (Korea Gas Corporation) Research Institute, Daegu, Republic of Korea, 4Center for Soft and Living Matter, Institute for Basic Science (IBS), UNIST-gil 50, Ulsan, Republic of Korea

JoVE 54143


 Bioengineering

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL, Universitat de Barcelona, L'Hospitalet de Llobregat, 2Institut de Neurociències, Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra

JoVE 55661


 Biochemistry

A RANKL-based osteoclast culture assay of mouse bone marrow to investigate the role of mTORC1 in osteoclast formation

1Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology, Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine

Video Coming Soon

JoVE 56468


 JoVE In-Press

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

1Plate-Forme d'Imagerie Dynamique, Imagopole, Institut Pasteur, 2Department of Radiation Oncology, Stanford School of Medicine, 3Service Hospitalier Frédéric Joliot, Institut d'Imagerie Biomédicale, 4Vanderbilt School of Medicine, 5The Walter & Eliza Hall Institute of Medical Research, 6Unité INSERM U786, Institut Pasteur, 7Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur

JoVE 51549


 Bioengineering

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

1Department of Microbiology, Biochemistry, Immunology, Morehouse School of Medicine, 2Department of Medicine, Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 4Yerkes National Primate Research Center

JoVE 53495


 Immunology and Infection

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

1Departments of Pathology and Infectious Diseases and Microbiology, University of Pittsburgh, 2University of Pittsburgh Cancer Institute, 3Department of Environmental and Occupational Health, University of Pittsburgh, 4Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences, University of Pittsburgh

JoVE 56001


 Cancer Research

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

1Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, 2Centre for Cancer Research & Cell Biology (CCRCB), School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast

Video Coming Soon

JoVE 56457


 JoVE In-Press

Establishment of a Clinic-based Biorepository

1Affiliated Dermatology & Affiliated Laboratories, Midwestern University Osteopathic Postdoctoral Training Institute, Midwestern University, 2Department of Microbiology & Immunology, Arizona College of Osteopathic Medicine, Midwestern University, 3Biomedical Sciences Program, College of Health Sciences, Midwestern University

JoVE 55583


 Medicine

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 2Division of Regenerative Medicine, Tulane National Primate Research Center, 3Department of Microbiology and Immunology, Tulane University School of Medicine, 4Department of Pharmacology, Tulane University School of Medicine

JoVE 50825


 Bioengineering

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

1Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 2Laboratory of Atherothrombosis, Cardiology Department, Moscow State University of Medicine and Dentistry, 3Sidra Medical and Research Center

JoVE 55020


 Immunology and Infection

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

Video Coming Soon

JoVE 56629


 JoVE In-Press

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